RAD 51稳定中性粒细胞胞外陷阱以分隔炎症
RAD51稳定中性粒细胞胞外陷阱以分隔炎症
作者： 小柯机器人 发布时间：2026/8/21 10:56:39
本期文章：《科学》：Volume 393 Issue 6813
英国弗朗西斯·克里克研究所Venizelos Papayannopoulos团队取得一项新突破。他们发现了RAD51稳定中性粒细胞胞外陷阱以分隔炎症。该研究于2026年8月20日发表于国际一流学术期刊《科学》杂志上。
该团队发现NET分支是由RAD51介导的，RAD51是一种在DNA重组修复过程中产生DNA连接的蛋白质。药物抑制、RAD51敲低或GEN1和RuvC溶解处理减少了神经网络的分支和不稳定，而RAD51在不同刺激下的上调产生的神经网络具有不同的稳定性。在小鼠肺部烟熏曲霉感染过程中，RAD51的抑制作用破坏了NETs，降低了肺细胞因子。
然而，循环中NET成分积累的增加导致循环单核细胞中白细胞介素-6（IL-6）的诱导，从而加剧了2型炎症和哮喘。人曲霉病细胞外血浆DNA与IL-6和嗜酸性粒细胞趋化因子相关。通过结构稳定NETs， RAD51区分炎症以阻止异常的全身免疫激活，将DNA修复与炎症联系起来。
据了解，中性粒细胞胞外陷阱（NETs）具有分支染色质结构，其起源和功能尚不清楚。
附：英文原文
Title: RAD51 stabilizes neutrophil extracellular traps to compartmentalize inflammation
Author: Lorenza Iolanda Tsansizi, Sophie Yihan Guan, Iker Valle Aramburu, Rajvee Shah Punatar, Thomas J. Williams, Yihe E. Qiao, Anna Reed, Darius Armstrong-James, Stephen C. West, Venizelos Papayannopoulos
Issue&Volume: 2026-08-20
Abstract: Neutrophil extracellular traps (NETs) feature a branched chromatin architecture whose origin and function remain unknown. We found that NET branching is mediated by RAD51, a protein generating DNA junctions during DNA recombination repair. Pharmacological inhibition, RAD51 knockdown, or GEN1 and RuvC resolvase treatment reduced branching and destabilized NETs, whereas RAD51 up-regulation by different stimuli generated NETs with variable stability. RAD51 inhibition during murine pulmonary Aspergillus fumigatus infection dismantled NETs and reduced lung cytokines. However, the increased accumulation of NET components in the circulation led to interleukin-6 (IL-6) induction in circulating monocytes that exacerbated type 2 inflammation and asthma. Extracellular plasma DNA correlated with IL-6 and eotaxin in human aspergillosis. By structurally stabilizing NETs, RAD51 compartmentalizes inflammation to thwart aberrant systemic immune activation, linking DNA repair to inflammation.
DOI: aed9286
Source: https://www.science.org/doi/10.1126/science.aed9286