从全能性到早期器官发生的灵长类胚胎发生的连续建模
从全能性到早期器官发生的灵长类胚胎发生的连续建模
作者： 小柯机器人 发布时间：2026/6/11 17:08:43
本期文章：《细胞》：Online/在线发表
近日，北京大学杜鹏团队研究出从全能性到早期器官发生的灵长类胚胎发生的连续建模。这一研究成果于2026年6月10日发表在国际顶尖学术期刊《细胞》上。
研究团队成功地从全能卵裂球样干细胞（cTBLCs）中获得了灵长类动物的器官发生胚胎。cTBLCs是由多能干细胞重新编程并稳定维持的，可以显示类似于受精卵/2 - 4细胞卵裂球的关键全能样分子和功能特征。cTBLCs自发地产生组织良好的囊胚，进一步从原肠胚形成发展到早期器官发生，它们表现出神经管样结构和心脏样跳动活动。
使用单细胞RNA测序（scRNA-seq），该团队可视化了从cTBLCs到有机遗传谱系的逐步细胞状态转变，并确定了中间的8细胞/样桑葚胚状态。有趣的是，研究组发现了一个源于ctblc的动态级联调控网络，它代表了一个内在的发育启动程序，独立于经典的受精相关事件。总之，cTBLC平台为研究发育起始、早期谱系规范和器官发生提供了一个统一的体外框架。
据介绍，胚胎样结构或胚状体是研究早期胚胎发育的有力模型。然而，以前的模型只涵盖了部分的发展阶段，限制了它们的应用。
附：英文原文
Title: Continuous modeling of primate embryogenesis from totipotency to early organogenesis
Author: Wei Zheng, Bing Peng, Zilin Chen, Kangwei Huang, Yueyao Qi, Huimin Niu, Hui Shen, Jun Wu, Jianwei Jiao, Peng Du
Issue&Volume: 2026-06-10
Abstract: Embryo-like structures, or embryoids, are powerful models to investigate early embryonic development. Yet, previous models cover only partial developmental stages, restricting their applications. Here, we successfully generate primate organogenetic embryoids from cynomolgus totipotent blastomere-like stem cells (cTBLCs). cTBLCs, reprogrammed from pluripotent stem cells and stably maintained, can display key totipotent-like molecular and functional features resembling zygotes/2–4-cell blastomeres. cTBLCs spontaneously generate well-organized blastoids that further progress through gastrulation to early organogenesis, thus exhibiting neural-tube-like structures and cardiac-like beating activity. Using single-cell RNA sequencing (scRNA-seq), we visualized stepwise cell-state transitions from cTBLCs to organogenetic lineages and identified an intermediate 8-cell/morula-like state. Interestingly, we uncovered a dynamic cascade of regulatory networks originating from cTBLCs, which represented an intrinsic developmental initiation program occurring independently of classical fertilization-associated events. Together, the cTBLC platform provides a unified in vitro framework to investigate developmental initiation, early lineage specification, and organogenesis.
DOI: 10.1016/j.cell.2026.05.023
Source: https://www.cell.com/cell/abstract/S0092-8674(26)00582-9