单细胞筛选平台加速植物功能遗传学

单细胞筛选平台加速植物功能遗传学

作者： 小柯机器人 发布时间：2026/5/6 15:40:06

本期文章：《自然—生物技术》：Online/在线发表

斯坦福大学Elizabeth S. Sattely团队在研究中取得进展。他们揭示了单细胞筛选平台加速植物功能遗传学。这一研究成果于2026年5月5日发表在国际顶尖学术期刊《自然—生物技术》上。

在这里，课题组报道了一个单细胞筛选平台，PIVOT（原生质体在植物中过表达后的分离），以加速植物基因的鉴定和功能表征。该课题组研究人员将本烟作为异源寄主来测试在单叶中排列的基因库。PIVOT利用病毒重复感染排除，以确保在池库传递过程中每个细胞的单一多重感染。此外，该课题组人员设计了一种细胞表面蛋白作为表型标记，用于从异质细胞群体中分离出感兴趣的细胞。利用该系统，课题组人员从拟南芥开放阅读框文库中恢复了细胞分裂素信号的调控因子。研究团队预计PIVOT将广泛应用于整个植物界的高通量、单细胞功能遗传筛选。

研究人员表示，利用现有的全植物遗传筛选工具，在模式和非模式植物中阐明高度冗余的遗传程序（如信号通路）中的基因功能是具有挑战性的。如果对携带遗传扰动的单个细胞进行主题化筛选，那么许多挑战都可以克服。

附：英文原文

Title: A single-cell screening platform accelerates functional genetics in plants

Author: Lowensohn, Tara N., Cody, Will B., Tsai, Chun, Vlahos, Alexander E., Call, Connor C., Gao, Xiaojing J., Sattely, Elizabeth S.

Issue&Volume: 2026-05-05

Abstract: Elucidating gene function in highly redundant genetic programs such as signaling pathways is challenging in model and nonmodel plants with current whole-plant genetic screening tools. Many of these challenges could be overcome if screens were instead carried out using individual cells harboring genetic perturbations. Here we report a single-cell screening platform, PIVOT (protoplast isolation after virus overexpression in planta), to accelerate identification and functional characterization of plant genes. We use Nicotiana benthamiana as a heterologous host to test gene libraries arrayed in a single leaf. PIVOT harnesses viral superinfection exclusion to ensure single multiplicity of infection per cell during pooled library delivery. Additionally, we engineer a cell-surface protein as a phenotypic marker for isolating cells of interest from a heterogeneous population. Using this system, we recover regulators of cytokinin signaling from an Arabidopsis open reading frame library. We anticipate PIVOT will be broadly applicable for high-throughput, single-cell functional genetic screening across the plant kingdom.

DOI: 10.1038/s41587-026-03094-4

Source: https://www.nature.com/articles/s41587-026-03094-4

主题：细胞